In contrast, a confocal microscope uses point illumination (see Point Spread Function) and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal – the name …

This paper lays out the basic principles, advantages, technical considerations, and applications for confocal microscopes to guide non-experts in determining the most appropriate confocal method for …

Coherent light emitted by the laser system (excitation source) passes through a pinhole aperture that is situated in a conjugate plane (confocal) with a scanning point on the specimen and a second pinhole …

Confocal microscopy is a type of fluorescence microscopy which uses a laser to excite fluorescence from fluorophores used to label different subsets of a specimen.

The confocal microscope was invented by Marvin Minsky, who has written a nice summary about this on the web. His original idea was to move the sample in order to scan it, rather than to use scanning …

Confocal microscopy offers several advantages over conventional optical microscopy, including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from …

Apr 11, 2024 · The concept of confocal microscopy was initially developed by Marvin Minsky in the 1950s, at Harvard University with an aim of viewing the neural network without staining the tissues …

Jan 16, 2025 · Confocal microscopes are essential tools in scientific research and industrial applications, offering unparalleled imaging precision and clarity. In this guide, we’ll explore what confocal …

Jul 30, 2025 · Confocal systems achieve their clarity by employing specialized components working together. A laser serves as the focused light source, providing an intense, highly directional beam …

The Laser Scanning Confocal Microscope (LSCM, or confocal) is a specialized type of light microscope. A laser beam is scanned across the sample to form the image, which is stored on a computer as a …